Introduction
We have designed a pipeline to identify conserved motifs and critical microRNAs using microRNA-TF-gene networks. It took us more than two months to go through the pipeline.
Now, we build a web server, named IOCMIN (Identification of Conserved Motifs in Networks), to do most of those work and it take only about 8 minutes.
Besides, IOCMIN has the following unique functions:
(i) it identifies the interactions among three kinds of biomolecules: miRNAs, TFs and genes (non-TFs);
(ii) it constructs regulatory networks;
(iii) it identifies hub nodes of networks and infers their potential functions by investigating GO and pathway enrichment of their targets;
(iv) it identifies three kinds of miRNA-TF-gene motifs;
(v) it identifies conserved regulatory interactions and networks across species.
Application Scenarios:
(i) Comparison of same disease among different species (e.g. human cleft palate vs. mouse cleft palate);
(ii) Comparison of different diseases (e.g. human cleft palate vs. human cleft lip);
(iii) Comparison of case vs. control. (e.g. human cleft palate vs. normal palate);
(iv) Comparison of same disease in different stages (e.g. mouse cleft palate gestation vs. postnatal stages);
(v) Comparison of different tissues (e.g. mouse palate vs. lip);
(vi) One species, build miRNA-TF-gene network (e.g. construct network for mouse cleft palate only);
(vii) Without miRNAs, build TF-gene network (e.g. construct TF-gene network without miRNAs).
News
(November 11, 2020) IOCMIN was launched.